Previous work has shown that the transport of some small protein cargoes through the nuclear pore complex (NPC) can occur in vitro in the absence of nucleoside triphosphate hydrolysis. We now demonstrate that in the importin α/β and transportin import pathways, efficient in vitro transport of large proteins, in contrast to smaller proteins, requires hydrolyzable GTP and the small GTPase Ran. Morphological and biochemical analysis indicates that the presence of Ran and GTP allows large cargo to efficiently cross central regions of the NPC. We further demonstrate that this function of RanGTP at least partly involves its direct binding to importin β and transportin. We suggest that RanGTP functions in these pathways to promote the transport of large cargo by enhancing the ability of import complexes to traverse diffusionally restricted areas of the NPC.
Skip Nav Destination
Article navigation
14 October 2002
Article|
October 07 2002
Influence of cargo size on Ran and energy requirements for nuclear protein import
Susan K. Lyman,
Susan K. Lyman
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Search for other works by this author on:
Tinglu Guan,
Tinglu Guan
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Search for other works by this author on:
Janna Bednenko,
Janna Bednenko
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Search for other works by this author on:
Harald Wodrich,
Harald Wodrich
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Search for other works by this author on:
Larry Gerace
Larry Gerace
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Search for other works by this author on:
Susan K. Lyman
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Tinglu Guan
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Janna Bednenko
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Harald Wodrich
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Larry Gerace
Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Address correspondence to Larry Gerace, Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037. Tel.: (858) 784-8514. Fax: (858) 784-9132. E-mail: [email protected]
*
Abbreviations used in this paper: βgal, β-galactosidase; FG, Phe-Gly; IBB, importin β binding domain; NPC, nuclear pore complex; Npl, nucleoplasmin core; NTP, nucleoside triphosphate; thyr, thyroglobulin; XTP, xanthosine triphosphate
Received:
April 30 2002
Revision Received:
August 30 2002
Accepted:
September 03 2002
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2002
J Cell Biol (2002) 159 (1): 55–67.
Article history
Received:
April 30 2002
Revision Received:
August 30 2002
Accepted:
September 03 2002
Citation
Susan K. Lyman, Tinglu Guan, Janna Bednenko, Harald Wodrich, Larry Gerace; Influence of cargo size on Ran and energy requirements for nuclear protein import . J Cell Biol 14 October 2002; 159 (1): 55–67. doi: https://doi.org/10.1083/jcb.200204163
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement