The authors were able to measure the diffusion of translocon complexes in the ER membrane by transfecting a temperature-sensitive mutant cell line with a GFP-tagged version of the translocon-associated oligosaccharyltransferase component Dad1. In cells that are actively carrying out translation, the diffusion constant of GFP-Dad1 is about seven...
The Rockefeller University Press
2002
The Rockefeller University Press
2002
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