β-Spectrin and ankyrin are major components of the membrane cytoskeleton. We have generated mice carrying a null mutation in the βIV-spectrin gene using gene trapping in embryonic stem cells. Mice homozygous for the mutation exhibit tremors and contraction of hindlimbs. βIV-spectrin expression is mostly restricted to neurons, where it colocalizes with and binds to ankyrin-G at axon initial segments (AISs) and nodes of Ranvier (NR). In βIV-spectrin–null neurons, neither ankyrin-G nor voltage-gated sodium channels (VGSC) are correctly clustered at these sites, suggesting that impaired action potential caused by mislocalization of VGSC leads to the phenotype. Conversely, in ankyrin-G–null neurons, βIV-spectrin is not localized to these sites. These results indicate that βIV-spectrin and ankyrin-G mutually stabilize the membrane protein cluster and the linked membrane cytoskeleton at AIS and NR.
βIV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier
- Views Icon Views
- Share Icon Share
- Search Site
Masayuki Komada, Philippe Soriano; βIV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier . J Cell Biol 21 January 2002; 156 (2): 337–348. doi: https://doi.org/10.1083/jcb.200110003
Download citation file: