We have investigated the requirements for NDJ1 in meiotic telomere redistribution and clustering in synchronized cultures of Saccharomyces cerevisiae. On induction of wild-type meiosis, telomeres disperse from premeiotic aggregates over the nuclear periphery, and then cluster near the spindle pole body (bouquet arrangement) before dispersing again. In ndj1Δ meiocytes, telomeres are scattered throughout the nucleus and fail to form perinuclear meiosis-specific distribution patterns, suggesting that Ndj1p may function to tether meiotic telomeres to the nuclear periphery. Since ndj1Δ meiocytes fail to cluster their telomeres at any prophase stage, Ndj1p is the first protein shown to be required for bouquet formation in a synaptic organism. Analysis of homologue pairing by two-color fluorescence in situ hybridization with cosmid probes to regions on III, IX, and XI revealed that disruption of bouquet formation is associated with a significant delay (>2 h) of homologue pairing. An increased and persistent fraction of ndj1Δ meiocytes with Zip1p polycomplexes suggests that chromosome polarization is important for synapsis progression. Thus, our observations support the hypothesis that meiotic telomere clustering contributes to efficient homologue alignment and synaptic pairing. Under naturally occurring conditions, bouquet formation may allow for rapid sporulation and confer a selective advantage.
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2 October 2000
Article|
October 02 2000
Meiotic Telomere Protein Ndj1p Is Required for Meiosis-Specific Telomere Distribution, Bouquet Formation and Efficient Homologue Pairing
Edgar Trelles-Sticken,
Edgar Trelles-Sticken
aDepartment of Human Biology and Genetics, University of Kaiserslautern, D-67653 Kaiserslautern, Germany
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Michael E. Dresser,
Michael E. Dresser
bProgram in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104
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Harry Scherthan
Harry Scherthan
aDepartment of Human Biology and Genetics, University of Kaiserslautern, D-67653 Kaiserslautern, Germany
cDepartment of Cell Biology, University of Kaiserslautern, D-67653 Kaiserslautern, Germany
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Edgar Trelles-Sticken
aDepartment of Human Biology and Genetics, University of Kaiserslautern, D-67653 Kaiserslautern, Germany
Michael E. Dresser
bProgram in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104
Harry Scherthan
aDepartment of Human Biology and Genetics, University of Kaiserslautern, D-67653 Kaiserslautern, Germany
cDepartment of Cell Biology, University of Kaiserslautern, D-67653 Kaiserslautern, Germany
Abbreviations used in this paper: cos, cosmid; FISH, fluorescence in situ hybridization; HA, hemagglutinin; IF, immunofluorescence; SPB, spindle pole body.
Received:
May 02 2000
Revision Requested:
August 22 2000
Accepted:
August 23 2000
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 2000 The Rockefeller University Press
2000
The Rockefeller University Press
J Cell Biol (2000) 151 (1): 95–106.
Article history
Received:
May 02 2000
Revision Requested:
August 22 2000
Accepted:
August 23 2000
Citation
Edgar Trelles-Sticken, Michael E. Dresser, Harry Scherthan; Meiotic Telomere Protein Ndj1p Is Required for Meiosis-Specific Telomere Distribution, Bouquet Formation and Efficient Homologue Pairing. J Cell Biol 2 October 2000; 151 (1): 95–106. doi: https://doi.org/10.1083/jcb.151.1.95
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