Mice incapable of synthesizing the abundant galactolipids of myelin exhibit disrupted paranodal axo-glial interactions in the central and peripheral nervous systems. Using these mutants, we have analyzed the role that axo-glial interactions play in the establishment of axonal protein distribution in the region of the node of Ranvier. Whereas the clustering of the nodal proteins, sodium channels, ankyrinG, and neurofascin was only slightly affected, the distribution of potassium channels and paranodin, proteins that are normally concentrated in the regions juxtaposed to the node, was dramatically altered. The potassium channels, which are normally concentrated in the paranode/juxtaparanode, were not restricted to this region but were detected throughout the internode in the galactolipid-defi- cient mice. Paranodin/contactin-associated protein (Caspr), a paranodal protein that is a potential neuronal mediator of axon-myelin binding, was not concentrated in the paranodal regions but was diffusely distributed along the internodal regions. Collectively, these findings suggest that the myelin galactolipids are essential for the proper formation of axo-glial interactions and demonstrate that a disruption in these interactions results in profound abnormalities in the molecular organization of the paranodal axolemma.
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13 December 1999
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December 13 1999
Axo-Glial Interactions Regulate the Localization of Axonal Paranodal Proteins
Jeffrey L. Dupree,
Jeffrey L. Dupree
aNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
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Jean-Antoine Girault,
Jean-Antoine Girault
dInstitut National de la Santé et la Recherche Médicale, Unit 114, Collège de France, Paris 75231, France
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Brian Popko
Brian Popko
aNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
bDepartment of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
cProgram in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
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Jeffrey L. Dupree
aNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Jean-Antoine Girault
dInstitut National de la Santé et la Recherche Médicale, Unit 114, Collège de France, Paris 75231, France
Brian Popko
aNeuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
bDepartment of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
cProgram in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Abbreviations used in this paper: Caspr, contactin-associated protein; CGT, ceramide galactosyltransferase; CNS, central nervous system; GalC, galactocerebroside; PB, phosphate buffer; PNS, peripheral nervous system.
Received:
October 12 1999
Revision Requested:
November 02 1999
Accepted:
November 03 1999
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 1999 The Rockefeller University Press
1999
The Rockefeller University Press
J Cell Biol (1999) 147 (6): 1145–1152.
Article history
Received:
October 12 1999
Revision Requested:
November 02 1999
Accepted:
November 03 1999
Citation
Jeffrey L. Dupree, Jean-Antoine Girault, Brian Popko; Axo-Glial Interactions Regulate the Localization of Axonal Paranodal Proteins. J Cell Biol 13 December 1999; 147 (6): 1145–1152. doi: https://doi.org/10.1083/jcb.147.6.1145
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