The warthog (wrt) gene, recovered as a modifier for Notch signaling, was found to encode the Drosophila homologue of rab6, Drab6. Vertebrate and yeast homologues of this protein have been shown to regulate Golgi network to TGN trafficking. To study the function of this protein in the development of a multicellular organism, we analyzed three different warthog mutants. The first was an R62C point mutation, the second a genomic null, and the third was an engineered GTP-bound form. Our studies show, contrary to yeast, that the Drosophila homologue of rab6 is an essential gene. However, it has limited effects on development beyond the larval stage. Only the mechanosensory bristles on the head, notum, and scutellum are affected by warthog mutations. We present models for the modifying effect of Drab6 on Notch signaling.
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23 August 1999
Article|
August 23 1999
The Developmental Role of warthog, the Notch Modifier Encoding Drab6
Karen Purcell,
Karen Purcell
aDepartment of Cell Biology, Yale University, New Haven, Connecticut 06510
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Spyros Artavanis-Tsakonas
Spyros Artavanis-Tsakonas
aDepartment of Cell Biology, Yale University, New Haven, Connecticut 06510
Search for other works by this author on:
Karen Purcell
aDepartment of Cell Biology, Yale University, New Haven, Connecticut 06510
Spyros Artavanis-Tsakonas
aDepartment of Cell Biology, Yale University, New Haven, Connecticut 06510
1.used in this paper: ORF, open reading frame; wrt, warthog
Received:
May 18 1999
Revision Requested:
July 01 1999
Accepted:
July 07 1999
Online ISSN: 1540-8140
Print ISSN: 0021-9525
© 1999 The Rockefeller University Press
1999
The Rockefeller University Press
J Cell Biol (1999) 146 (4): 731–740.
Article history
Received:
May 18 1999
Revision Requested:
July 01 1999
Accepted:
July 07 1999
Citation
Karen Purcell, Spyros Artavanis-Tsakonas; The Developmental Role of warthog, the Notch Modifier Encoding Drab6. J Cell Biol 23 August 1999; 146 (4): 731–740. doi: https://doi.org/10.1083/jcb.146.4.731
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