Pollen tube cells elongate based on actin- dependent targeted secretion at the tip. Rho family small GTPases have been implicated in the regulation of related processes in animal and yeast cells. We have functionally characterized Rac type Rho family proteins that are expressed in growing pollen tubes. Expression of dominant negative Rac inhibited pollen tube elongation, whereas expression of constitutive active Rac induced depolarized growth. Pollen tube Rac was found to accumulate at the tip plasma membrane and to physically associate with a phosphatidylinositol monophosphate kinase (PtdIns P-K) activity. Phosphatidylinositol 4, 5-bisphosphate (PtdIns 4, 5-P2), the product of PtdIns P-Ks, showed a similar intracellular localization as Rac. Expression of the pleckstrin homology (PH)-domain of phospholipase C (PLC)-δ1, which binds specifically to PtdIns 4, 5-P2, inhibited pollen tube elongation. These results indicate that Rac and PtdIns 4, 5-P2 act in a common pathway to control polar pollen tube growth and provide direct evidence for a function of PtdIns 4, 5-P2 compartmentalization in the regulation of this process.
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19 April 1999
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April 19 1999
Rac Homologues and Compartmentalized Phosphatidylinositol 4, 5-Bisphosphate Act in a Common Pathway to Regulate Polar Pollen Tube Growth
Benedikt Kost,
Benedikt Kost
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
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Emmanuel Lemichez,
Emmanuel Lemichez
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
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Pius Spielhofer,
Pius Spielhofer
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
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Yan Hong,
Yan Hong
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
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Kimberly Tolias,
Kimberly Tolias
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
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Christopher Carpenter,
Christopher Carpenter
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
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Nam-Hai Chua
Nam-Hai Chua
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
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Benedikt Kost
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
Emmanuel Lemichez
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
Pius Spielhofer
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
Yan Hong
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
Kimberly Tolias
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
Christopher Carpenter
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
Nam-Hai Chua
*Laboratory of Plant Molecular Biology, The Rockefeller University, New York 10021-6399; ‡Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604; §Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02115; and ‖Department of Cell Biology and ¶Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215
Address correspondence to Nam-Hai Chua, Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Ave., New York, NY 10021-6399. Tel.: (212) 327-8126. Fax: (212) 327-8327. E-mail: [email protected]
The work presented here was supported by the following organizations: DOE (grant DOE94ER20143 to N.-H. Chua), Swiss National Science Foundation (grants 823A-050394 and 823A-046686 to P. Spielhofer and B. Kost), Human Frontier Science Program (grant LT-256/97 to E. Lemichez), and the National Institutes of Health (grant GM54389 to C. Carpenter).
Benedikt Kost's present address is Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore 117604.
Received:
January 05 1999
Revision Received:
March 15 1999
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1999
J Cell Biol (1999) 145 (2): 317–330.
Article history
Received:
January 05 1999
Revision Received:
March 15 1999
Citation
Benedikt Kost, Emmanuel Lemichez, Pius Spielhofer, Yan Hong, Kimberly Tolias, Christopher Carpenter, Nam-Hai Chua; Rac Homologues and Compartmentalized Phosphatidylinositol 4, 5-Bisphosphate Act in a Common Pathway to Regulate Polar Pollen Tube Growth . J Cell Biol 19 April 1999; 145 (2): 317–330. doi: https://doi.org/10.1083/jcb.145.2.317
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