A cholera toxin mutant (CTX–K63) unable to raise cAMP levels was used to study in Vero cells the retrograde transport of the toxin A subunit (CTX-A–K63), which possesses a COOH-terminal KDEL retrieval signal. Microinjected GTP-γ-S inhibits the internalization as well as Golgi–ER transport of CTX-A–K63. The appearance of CTX-A–K63 in the Golgi induces a marked dispersion of Erd2p and p53 but not of the Golgi marker giantin. Erd2p is translocated under these conditions most likely to the intermediate compartment as indicated by an increased colocalization of Erd2p with mSEC13, a member of the mammalian coat protein II complex. IgGs as well as Fab fragments directed against Erd2p, β-COP, or p23, a new member of the p24 protein family, inhibit or block retrograde transport of CTX-A–K63 from the Golgi without affecting its internalization or its transport to the Golgi. Anti-Erd2p antibodies do not affect the binding of CTX-A to Erd2p, but inhibit the CTX-K63–induced translocation of Erd2p and p53.
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2 November 1998
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November 02 1998
KDEL Receptor (Erd2p)-mediated Retrograde Transport of the Cholera Toxin A Subunit from the Golgi Involves COPI, p23, and the COOH Terminus of Erd2p
Irina Majoul,
Irina Majoul
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
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Kai Sohn,
Kai Sohn
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
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Felix Theodor Wieland,
Felix Theodor Wieland
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
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Rainer Pepperkok,
Rainer Pepperkok
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
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Mariagrazia Pizza,
Mariagrazia Pizza
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
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Jörg Hillemann,
Jörg Hillemann
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
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Hans-Dieter Söling
Hans-Dieter Söling
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
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Irina Majoul
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
Kai Sohn
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
Felix Theodor Wieland
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
Rainer Pepperkok
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
Mariagrazia Pizza
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
Jörg Hillemann
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
Hans-Dieter Söling
*Abteilung Klinische Biochemie, Universität Göttingen, D-37070 Göttingen, Germany; ‡Institut für Biochemie I, University of Heidelberg, D-69120 Heidelberg, Germany; §European Molecular Biology Laboratory, D-69012 Heidelberg, Germay; ‖IRIS, The Chiron Vaccines Immunobiological Research Institute, Via Fiorentina, I-53100 Siena, Italy; and ¶Max-Planck-Institut für Biophysikalische Chemie, D-37077 Göttingen, Germany
Address correspondence to Hans-Dieter Söling, Max-Planck-Institut für Biophysikalische Chemie, Am Faβberg, D-37077 Göttingen, Germany. Tel.: (49) 201-1617. Fax: (49) 201-1639. E-mail: [email protected]
Received:
December 15 1997
Revision Received:
August 24 1998
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1998
J Cell Biol (1998) 143 (3): 601–612.
Article history
Received:
December 15 1997
Revision Received:
August 24 1998
Citation
Irina Majoul, Kai Sohn, Felix Theodor Wieland, Rainer Pepperkok, Mariagrazia Pizza, Jörg Hillemann, Hans-Dieter Söling; KDEL Receptor (Erd2p)-mediated Retrograde Transport of the Cholera Toxin A Subunit from the Golgi Involves COPI, p23, and the COOH Terminus of Erd2p . J Cell Biol 2 November 1998; 143 (3): 601–612. doi: https://doi.org/10.1083/jcb.143.3.601
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