A set of nuclear mutants of C. reinhardtii were identified that specifically lack translation of the chloroplast-encoded psbA mRNA, which encodes the photosystem II reaction center polypeptide D1. Two of these mutants are deficient in the 47-kD member (RB47) of the psbA RNA-binding complex, which has previously been identified both genetically and biochemically as a putative translational activator of the chloroplast psbA mRNA. RB47 is a member of the poly(A)-binding protein family, and binds with high affinity and specificity to the 5′ untranslated region of the psbA mRNA. The results presented here confirm RB47's role as a message-specific translational activator in the chloroplast, and bring together genetic and biochemical data to form a cohesive model for light- activated translational regulation in the chloroplast.
Translation of the Chloroplast psbA mRNA Requires the Nuclear-encoded Poly(A)-binding Protein, RB47
C.B. Yohn's present address is Program in Developmental Genetics, The Skirball Institute, New York University Medical Center, 540 First Avenue, 4th Floor, New York, NY 10016.
Address all correspondence to Stephen P. Mayfield, Department of Cell Biology, and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037. Tel.: (619) 784-9848. Fax: (619) 784-9840. E-mail: [email protected]
Christopher B. Yohn, Amybeth Cohen, Cristen Rosch, Michael R. Kuchka, Stephen P. Mayfield; Translation of the Chloroplast psbA mRNA Requires the Nuclear-encoded Poly(A)-binding Protein, RB47 . J Cell Biol 27 July 1998; 142 (2): 435–442. doi: https://doi.org/10.1083/jcb.142.2.435
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