Selective Entrapment of Extrachromosomally Amplified DNA by Nuclear Budding and Micronucleation during S Phase

We would like to acknowledge S.P. Linke (National Cancer Institute, Bethesda, MD) for kindly providing cell lines, D. Peterson (Salk Institute, La Jolla, CA) for his kind help on the operation of confocal microscopy that appeared in Fig. 6, and D. Von Hoff and K. Davidson (both from Institute of Drug Development, University of Texas Health Science Center, San Antonio, TX) for allowing us to cite their unpublished results. We thank T. Shimura and N. Kumon (both from Hiroshima University, Higashi-Hiroshima, Japan) for their technical help. T. Paulson, T. Kanda, S. O'Gorman, S. Pfaff, G. Karpen, O. Vafa, and L.-c. Huang (all from Salk Institute except Paulson [Fred Hutchinson Cancer Research Center, Seattle, WA] and Huang [UBI, Menlo Park, CA]) provided helpful discussions concerning experimental procedures and topics presented in this manuscript.

This work was in part supported by a grant from the United States Department of Army, (grant number DAMD17-94-J4359) and the International Collaboration Grant from Japanese Ministry of Education (grant number 07044271), and the G. Harold and Leila Y. Mathers Charitable Foundation.

Address all correspondence to Geoffrey Wahl, Gene Expression Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037. Tel: (619) 453-4100. Fax: (619) 552-8285. E-mail: [email protected]