CAS/Crk Coupling Serves as a “Molecular Switch” for Induction of Cell Migration

1. Abbreviations used in this paper: aa, amino acid(s); CAS, Crk-associated substrate; ECM, extracellular matrix; FAK, focal adhesion kinase; FBM, fibroblast basal medium; IGF-1, insulin-like growth factor 1; SH, src homology.

The authors would like to thank Drs. A. Minden and M. Karin (University of California, San Diego, CA) for providing Rac and Ras constructs. We are also grateful to Dr. M. Matsuda (International Medical Center of Japan, Tokyo, Japan) for original Crk constructs, and Drs. H. Hirai (University of Tokyo, Tokyo, Japan) and B. Mayer (Howard Hughes Medical Institute, The Children's Hospital, Boston, MA) for CAS cDNA constructs and expression vectors.

D.A. Cheresh was supported by grants HL-54444, CA-50286, and CA-45726 from the National Institutes of Health (NIH). R.L. Klemke was supported by a fellowship award from the Joseph Drown Foundation. J. Leng was supported by the U.S. Army Medical Research and Material Command under DAMD17-96-1-6104. K. Vuori was supported by grant CA-71560 from the NIH. K. Vuori is a PEW scholar in Biomedical Sciences. This manuscript is number 11052-IMM from The Scripps Research Institute.

Address all correspondence to Richard L. Klemke, Departments of Immunology and Vascular Biology, IMM-24, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Tel.: (619) 784-8164. Fax: 619-784-8926. E-mail: [email protected]