Thy-1, a glycosylphosphatidylinositol-linked integral membrane protein of the immunoglobulin superfamily, is a component of both large dense-core and small clear vesicles in PC12 cells. A majority of this protein, formerly recognized only on the plasma membrane of neurons, is localized to regulated secretory vesicles. Thy-1 is also present in synaptic vesicles in rat central nervous system. Experiments on permeabilized PC12 cells demonstrate that antibodies against Thy-1 inhibit the regulated release of neurotransmitter; this inhibition appears to be independent of any effect on the Ca2+ channel. These findings suggest Thy-1 is an integral component of many types of regulated secretory vesicles, and plays an important role in the regulated vesicular release of neurotransmitter at the synapse.
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9 February 1998
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February 09 1998
Thy-1 Is a Component Common to Multiple Populations of Synaptic Vesicles
Chung-Jiuan Jeng,
Chung-Jiuan Jeng
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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Steven A. McCarroll,
Steven A. McCarroll
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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Thomas F. J. Martin,
Thomas F. J. Martin
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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Erik Floor,
Erik Floor
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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James Adams,
James Adams
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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David Krantz,
David Krantz
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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Stefan Butz,
Stefan Butz
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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Robert Edwards,
Robert Edwards
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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Erik S. Schweitzer
Erik S. Schweitzer
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
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Chung-Jiuan Jeng
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
Steven A. McCarroll
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
Thomas F. J. Martin
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
Erik Floor
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
James Adams
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
David Krantz
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
Stefan Butz
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
Robert Edwards
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
Erik S. Schweitzer
*Department of Neurobiology and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90095-1763; ‡Departments of Neurology and Physiology, and Graduate Program in Neuroscience and Cell Biology, UCSF School of Medicine, San Francisco, California 94143-0435; §Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706-1569; ‖Department of Biochemistry, Cell & Molecular Biology, University of Kansas, Lawrence, Kansas 66045-2106; and ¶Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75235
Address all correspondence to Erik Schweitzer, Department of Neurobiology, UCLA School of Medicine, 10833 LeConte Avenue, Los Angeles, CA 90095-1763. Tel.: (310) 206-4084. Fax: (310) 206-4066. E-mail: [email protected]
Received:
August 20 1997
Revision Received:
December 08 1997
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1998
J Cell Biol (1998) 140 (3): 685–698.
Article history
Received:
August 20 1997
Revision Received:
December 08 1997
Citation
Chung-Jiuan Jeng, Steven A. McCarroll, Thomas F. J. Martin, Erik Floor, James Adams, David Krantz, Stefan Butz, Robert Edwards, Erik S. Schweitzer; Thy-1 Is a Component Common to Multiple Populations of Synaptic Vesicles . J Cell Biol 9 February 1998; 140 (3): 685–698. doi: https://doi.org/10.1083/jcb.140.3.685
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