Unfixed, compressed acinar cells of rat pancreas, isolated by mechanical and enzymatic means, were examined by phase microscopy and photomicrographed using 35 mm film and electronic flash illumination. Similarly, observations were made on Walker carcinoma cells; in addition, these cells were treated with solutions containing either phosphatidase A or enzyme inhibitors. Acinar cells contained, besides nuclei, perinuclear droplets and secretion granules, various membranous and vacuolar structures. The basal cytoplasm showed parallel dark lines interpreted as endoplasmic reticulum. In some cells, fragmentation of the reticulum was followed by the direct incorporation of fragments into simple myelin figures. In other cells it appeared that phase-lucent linear structures and vacuoles were derived by dilatation of cisternae of the endoplasmic reticulum. Perinuclear fluid collections arose either by dilation of the perinuclear cisternae of the endoplasmic reticulum or by fluid dilatation of the nuclear envelope. Phosphatidase A disrupted early vacuoles of Walker carcinoma cells. From this and the direct involvement of elements of the endoplasmic reticulum in myelin figures, it was concluded that the membranes limiting the endoplasmic reticulum incorporate phosphatides in continuous layers. While many severely injured cells formed large vacuoles, others developed concentrically laminated myelin figures; it was concluded that both types of structure derived from phosphatides liberated intracellularly, the vacuoles by vesicular myelin figure formation.
Skip Nav Destination
Article navigation
1 September 1962
Article|
September 01 1962
CELLULAR INJURY IN VITRO: PHASE CONTRAST STUDIES ON INJURED CYTOPLASM
I. K. Buckley
I. K. Buckley
From the Department of Pathology, University of Melbourne, Australia
Search for other works by this author on:
I. K. Buckley
From the Department of Pathology, University of Melbourne, Australia
Received:
June 06 1961
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright, 1962, by The Rockefeller Institute Press
1962
J Cell Biol (1962) 14 (3): 401–420.
Article history
Received:
June 06 1961
Citation
I. K. Buckley; CELLULAR INJURY IN VITRO: PHASE CONTRAST STUDIES ON INJURED CYTOPLASM . J Cell Biol 1 September 1962; 14 (3): 401–420. doi: https://doi.org/10.1083/jcb.14.3.401
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement