Centromere-associated protein E (CENP-E) is a kinesin-related microtubule motor protein that is essential for chromosome congression during mitosis. Using immunoelectron microscopy, CENP-E is shown to be an integral component of the kinetochore corona fibers that tether centromeres to the spindle. Immediately upon nuclear envelope fragmentation, an associated plus end motor trafficks cytoplasmic CENP-E toward chromosomes along astral microtubules that enter the nuclear volume. Before or concurrently with initial lateral attachment of spindle microtubules, CENP-E targets to the outermost region of the developing kinetochores. After stable attachment, throughout chromosome congression, at metaphase, and throughout anaphase A, CENP-E is a constituent of the corona fibers, extending at least 50 nm away from the kinetochore outer plate and intertwining with spindle microtubules. In congressing chromosomes, CENP-E is preferentially associated with (or accessible at) the stretched, leading kinetochore known to provide the primary power for chromosome movement. Taken together, this evidence strongly supports a model in which CENP-E functions in congression to tether kinetochores to the disassembling microtubule plus ends.
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20 October 1997
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October 20 1997
The Microtubule-dependent Motor Centromere–associated Protein E (CENP-E) Is an Integral Component of Kinetochore Corona Fibers That Link Centromeres to Spindle Microtubules
Xuebiao Yao,
Xuebiao Yao
*Laboratory of Cell Biology, Ludwig Institute for Cancer Research, ‡Division of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093-0660
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Karen L. Anderson,
Karen L. Anderson
*Laboratory of Cell Biology, Ludwig Institute for Cancer Research, ‡Division of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093-0660
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Don W. Cleveland
Don W. Cleveland
*Laboratory of Cell Biology, Ludwig Institute for Cancer Research, ‡Division of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093-0660
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Xuebiao Yao
*Laboratory of Cell Biology, Ludwig Institute for Cancer Research, ‡Division of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093-0660
Karen L. Anderson
*Laboratory of Cell Biology, Ludwig Institute for Cancer Research, ‡Division of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093-0660
Don W. Cleveland
*Laboratory of Cell Biology, Ludwig Institute for Cancer Research, ‡Division of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093-0660
Address all correspondence to Dr. Don W. Cleveland, 3080 CMM-East, University of California, 9500 Gilman Drive, La Jolla, CA 92093-0660. Tel.: (619) 534-7811. Fax: (619) 534-7659.
1. Abbreviation used in this paper: CENP, centromere-associated protein.
Received:
June 20 1997
Revision Received:
July 31 1997
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1997
J Cell Biol (1997) 139 (2): 435–447.
Article history
Received:
June 20 1997
Revision Received:
July 31 1997
Citation
Xuebiao Yao, Karen L. Anderson, Don W. Cleveland; The Microtubule-dependent Motor Centromere–associated Protein E (CENP-E) Is an Integral Component of Kinetochore Corona Fibers That Link Centromeres to Spindle Microtubules . J Cell Biol 20 October 1997; 139 (2): 435–447. doi: https://doi.org/10.1083/jcb.139.2.435
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