By immunoaffinity purification with the mAb Lan3-2, we have identified two novel Ig superfamily members, Tractin and LeechCAM. LeechCAM is an NCAM/FasII/ApCAM homologue, whereas Tractin is a cleaved protein with several unique features that include a PG/YG repeat domain that may be part of or interact with the extracellular matrix. Tractin and LeechCAM are widely expressed neural proteins that are differentially glycosylated in sets and subsets of peripheral sensory neurons that form specific fascicles in the central nervous system. In vivo antibody perturbation of the Lan3-2 glycoepitope demonstrates that it can selectively regulate extension of neurites and filopodia. Thus, these experiments provide evidence that differential glycosylation can confer functional diversity and specificity to widely expressed neural proteins.
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14 July 1997
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July 14 1997
Differential Glycosylation of Tractin and LeechCAM, Two Novel Ig Superfamily Members, Regulates Neurite Extension and Fascicle Formation
Yueqiao Huang,
Yueqiao Huang
*Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011; and ‡Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008
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John Jellies,
John Jellies
*Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011; and ‡Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008
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Kristen M. Johansen,
Kristen M. Johansen
*Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011; and ‡Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008
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Jørgen Johansen
Jørgen Johansen
*Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011; and ‡Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008
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Yueqiao Huang
*Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011; and ‡Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008
John Jellies
*Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011; and ‡Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008
Kristen M. Johansen
*Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011; and ‡Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008
Jørgen Johansen
*Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011; and ‡Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008
1. Abbreviations used in this paper: CAM, cellular adhesion molecule; CNS, central nervous system; FNIII, fibronectin type III.
Please address all correspondence to Jørgen Johansen, Department of Zoology and Genetics, 3156 Molecular Biology Building, Iowa State University, Ames, IA 50011. Tel.: (515) 294-2358. Fax: (515) 294-0345. e-mail: [email protected]
Received:
March 27 1997
Revision Received:
May 09 1997
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1997
J Cell Biol (1997) 138 (1): 143–157.
Article history
Received:
March 27 1997
Revision Received:
May 09 1997
Citation
Yueqiao Huang, John Jellies, Kristen M. Johansen, Jørgen Johansen; Differential Glycosylation of Tractin and LeechCAM, Two Novel Ig Superfamily Members, Regulates Neurite Extension and Fascicle Formation. J Cell Biol 14 July 1997; 138 (1): 143–157. doi: https://doi.org/10.1083/jcb.138.1.143
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