p53/58 is a transmembrane protein that continuously recycles between the ER and pre-Golgi intermediates composed of vesicular-tubular clusters (VTCs) found in the cell periphery and at the cis face of the Golgi complex. We have generated an antibody that uniquely recognizes the p53/58 cytoplasmic tail. Here we present evidence that this antibody arrests the anterograde transport of vesicular stomatitis virus glycoprotein and leads to the accumulation of p58 in preGolgi intermediates. Consistent with a role for the KKXX retrieval motif found at the cytoplasmic carboxyl terminus of p53/58 in retrograde traffic, inhibition of transport through VTCs correlates with the ability of the antibody to block recruitment of COPI coats to the p53/58 cytoplasmic tail and to p53/58-containing membranes. We suggest that p53/58 function may be required for the coupled exchange of COPII for COPI coats during segregation of anterograde and retrograde transported proteins.
p53/58 Binds COPI and Is Required for Selective Transport through the Early Secretory Pathway
1. Abbreviations used in this paper: endo D and H, endoglycosidase D and H; GST, glutathione-S-transferase; NRK, normal rat kidney; VSV-G, vesicular stomatitis glycoprotein; VTC, vesicular tubular cluster.
Address all correspondence to William E. Balch, The Scripps Research Institute, Departments of Cell and Molecular Biology, IMM-11, 10550 N. Torrey Pines Road, La Jolla, CA 92037. Tel.: (619) 784-2310. Fax: (619) 784-9126. E-mail: [email protected]
Ellen J. Tisdale's current address is Wayne State University, School of Medicine, Department of Pharmacology, 540 East Canfield, Detroit, MI 48201-1908.
Ellen J. Tisdale, Helen Plutner, Jeanne Matteson, William E. Balch; p53/58 Binds COPI and Is Required for Selective Transport through the Early Secretory Pathway. J Cell Biol 5 May 1997; 137 (3): 581–593. doi: https://doi.org/10.1083/jcb.137.3.581
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