RPTP mu is a receptor-like protein tyrosine phosphatase that mediates homophilic cell-cell interactions. Surface expression of RPTP mu is restricted to cell-cell contacts and is upregulated with increasing cell density, suggesting a role for RPTP mu in contact-mediated signaling. It was recently reported (Brady-Kalnay, S.M., D.L. Rimm, and N.K. Tonks. 1995. J. Cell Biol. 130:977-986) that RPTP mu binds directly to cadherin/catenin complexes, and thus may regulate the tyrosine phosphorylation of such complexes. Here we report that this concept needs revision. Through reciprocal precipitations using a variety of antibodies against RPTP mu, cadherins, and catenins, we show that RPTP mu does not interact with cadherin/catenin complexes, even when assayed under very mild lysis conditions. We find that the anti-RPTP mu antiserum used by others precipitates cadherins in a nonspecific manner independent of RPTP mu. We conclude that, contrary to previous claims, RPTP mu does not interact with cadherin complexes and thus is unlikely to directly regulate cadherin/catenin function.
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15 September 1996
Article|
September 15 1996
Lack of association between receptor protein tyrosine phosphatase RPTP mu and cadherins.
G C Zondag,
G C Zondag
Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
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W H Moolenaar,
W H Moolenaar
Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
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M F Gebbink
M F Gebbink
Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
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G C Zondag
Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
W H Moolenaar
Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
M F Gebbink
Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1996) 134 (6): 1513–1517.
Citation
G C Zondag, W H Moolenaar, M F Gebbink; Lack of association between receptor protein tyrosine phosphatase RPTP mu and cadherins.. J Cell Biol 15 September 1996; 134 (6): 1513–1517. doi: https://doi.org/10.1083/jcb.134.6.1513
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