Members of the rab/YPT1/SEC4 gene family of small molecular weight GTPases play key roles in the regulation of vesicular traffic between compartments of the exocytic pathway. Using immunoelectron microscopy, we demonstrate that a dominant negative rab1a mutant, rab1a(N124I), defective for guanine nucleotide binding in vitro, leads to the accumulation of vesicular stomatitis virus glycoprotein (VSV-G) in numerous pre-cis-Golgi vesicles and vesicular-tubular clusters containing rab1 and beta-COP, a subunit of the coatomer complex. Similar to previous observations (Balch et al. 1994. Cell. 76:841-852), VSV-G was concentrated nearly 5-10-fold in vesicular carriers that accumulate in the presence of the rab1a(N124I) mutant. VSV-G containing vesicles and vesicular-tubular clusters were also found to accumulate in the presence of a rab1a effector domain peptide mimetic that inhibits endoplasmic reticulum to Golgi transport, as well as in the absence of Ca2+. These results suggest that the combined action of a Ca(2+)-dependent protein and conformational changes associated with the GTPase cycle of rab1 are essential for a late targeting/fusion step controlling the delivery of vesicles to Golgi compartments.
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15 April 1994
Article|
April 15 1994
Rab1 and Ca2+ are required for the fusion of carrier vesicles mediating endoplasmic reticulum to Golgi transport.
S N Pind,
S N Pind
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
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C Nuoffer,
C Nuoffer
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
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J M McCaffery,
J M McCaffery
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
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H Plutner,
H Plutner
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
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H W Davidson,
H W Davidson
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
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M G Farquhar,
M G Farquhar
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
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W E Balch
W E Balch
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
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S N Pind
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
C Nuoffer
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
J M McCaffery
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
H Plutner
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
H W Davidson
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
M G Farquhar
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
W E Balch
Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1994) 125 (2): 239–252.
Citation
S N Pind, C Nuoffer, J M McCaffery, H Plutner, H W Davidson, M G Farquhar, W E Balch; Rab1 and Ca2+ are required for the fusion of carrier vesicles mediating endoplasmic reticulum to Golgi transport.. J Cell Biol 15 April 1994; 125 (2): 239–252. doi: https://doi.org/10.1083/jcb.125.2.239
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