An Mr 63-kD sea urchin sperm flagellar membrane protein has been previously implicated as a possible receptor for egg jelly ligand(s) that trigger the sperm acrosome reaction (AR). The cDNA and deduced amino acid sequences of the 63-kD protein are presented. The open reading frame codes for a protein of 470 amino acids which contains a putative signal sequence of 25 residues. Western blots using antibodies to two synthetic peptides confirm the sequence to be that of the 63-kD protein. The mRNA is approximately 2,300 bases in length and the gene appears to be single copy. The protein is released from sperm membrane vesicles by treatment with phosphatidylinositol-specific phospholipase C, showing that it is anchored to the flagellar membrane by glycosylphosphatidyl inositol (GPI). Although we cannot demonstrate involvement of the 63-kD protein in the AR, it is of potential interest because it shares significant similarity with the developmentally expressed proteins crumbs, notch and xotch as well as human uromodulin over a region that includes two separate EGF repeats.
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15 June 1993
Article|
June 15 1993
A GPI-anchored sea urchin sperm membrane protein containing EGF domains is related to human uromodulin.
L M Mendoza,
L M Mendoza
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
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D Nishioka,
D Nishioka
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
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V D Vacquier
V D Vacquier
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
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L M Mendoza
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
D Nishioka
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
V D Vacquier
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla 92093-0202.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1993) 121 (6): 1291–1297.
Citation
L M Mendoza, D Nishioka, V D Vacquier; A GPI-anchored sea urchin sperm membrane protein containing EGF domains is related to human uromodulin.. J Cell Biol 15 June 1993; 121 (6): 1291–1297. doi: https://doi.org/10.1083/jcb.121.6.1291
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