We have examined the expression of brain-specific tropomyosins during neuronal differentiation. Both TmBr-1 and TmBr-3 were shown to be neuron specific. TmBr-1 and TmBr-3 mRNA levels increased during the most active phase of neurite outgrowth in the developing rat cerebellum. In PC12 cells stimulated by nerve growth factor (NGF) to differentiate to the neuronal phenotype, TmBr-1 and TmBr-3 levels increased with an increasing degree of morphological differentiation. Induction of TmBr-1 and TmBr-3 expression only occurred under conditions where PC12 cells were permitted to extend neurites. NGF was unable to maintain levels of TmBr-1 and TmBr-3 with the loss of neuronal phenotype by resuspension of differentiated PC12 cells. The unique cellular expression and regulation in vivo and in vitro of TmBr-1 and TmBr-3 strongly suggests a critical role of these tropomyosins in neuronal microfilament function. The findings reveal that the induction and maintenance of the neuronal tropomyosins is dependent on morphological differentiation and the maintenance of the neuronal phenotype.
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1 January 1993
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January 01 1993
Induction of neuron-specific tropomyosin mRNAs by nerve growth factor is dependent on morphological differentiation.
R P Weinberger,
R P Weinberger
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
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R C Henke,
R C Henke
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
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O Tolhurst,
O Tolhurst
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
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P L Jeffrey,
P L Jeffrey
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
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P Gunning
P Gunning
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
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R P Weinberger
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
R C Henke
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
O Tolhurst
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
P L Jeffrey
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
P Gunning
Developmental Neurobiology Unit, Children's Medical Research Institute, Wentworthville, N.S.W., Australia.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1993) 120 (1): 205–215.
Citation
R P Weinberger, R C Henke, O Tolhurst, P L Jeffrey, P Gunning; Induction of neuron-specific tropomyosin mRNAs by nerve growth factor is dependent on morphological differentiation.. J Cell Biol 1 January 1993; 120 (1): 205–215. doi: https://doi.org/10.1083/jcb.120.1.205
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