In this study, the putative laminin receptor function of the alpha 6 beta 4 integrin was assessed. For this purpose, we used a human cell line, referred to as clone A, that was derived from a highly invasive, colon adenocarcinoma. This cell line, which expresses the alpha 6 beta 4 integrin, adheres to the E8 and not to the P1 fragment of laminin. The adhesion of clone A cells to laminin is extremely rapid with half-maximal adhesion observed at 5 min after plating. Adhesion to laminin is blocked by GoH3, and alpha 6 specific antibody (60% inhibition), as well as by A9, a beta 4 specific antibody (30% inhibition). Most importantly, we demonstrate that alpha 6 beta 4 binds specifically to laminin-Sepharose columns in the presence of either Mg2+ or Mn2+ and it is eluted from these columns with EDTA but not with NaCl. The alpha 6 beta 4 integrin does not bind to collagen-Sepharose, but the alpha 2 beta 1 integrin does bind. Clone A cells do not express alpha 6 beta 1 as evidenced by the following observations: (a) no beta 1 integrin is detected in beta 1 immunoblots of GoH3 immunoprecipitates; and (b) no alpha 6 beta 1 integrin is seen in GoH3 immunoprecipitates of clone A extracts that had been immunodepleted of all beta 4 containing integrin using the A9 antibody. These data establish that laminin is a ligand for the alpha 6 beta 4 integrin and that this integrin can function as a laminin receptor independently of alpha 6 beta 1.
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1 May 1992
Article|
May 01 1992
The integrin alpha 6 beta 4 is a laminin receptor.
E C Lee,
E C Lee
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
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M M Lotz,
M M Lotz
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
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G D Steele, Jr,
G D Steele, Jr
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
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A M Mercurio
A M Mercurio
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
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E C Lee
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
M M Lotz
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
G D Steele, Jr
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
A M Mercurio
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1992) 117 (3): 671–678.
Citation
E C Lee, M M Lotz, G D Steele, A M Mercurio; The integrin alpha 6 beta 4 is a laminin receptor.. J Cell Biol 1 May 1992; 117 (3): 671–678. doi: https://doi.org/10.1083/jcb.117.3.671
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