We have isolated a protein from media conditioned by a murine marrow-derived cell line (PB6) and from mouse marrow supernatants that antagonizes interleukin 3-dependent proliferation of cells in culture and reversibly inhibits DNA synthesis of erythroid progenitor cells (BFU-E) in vitro. This protein, p16 (monomer Mr = 16 kD on SDS-PAGE), was purified to homogeneity and amino acid sequencing of a polypeptide fragment yielded a sequence identical to that of murine cytosolic Cu,Zn-containing superoxide dismutase (SOD). The identification of p16 as SOD was confirmed by the detection of SOD enzymatic activity in pure p16 fractions, and when a commercial human erythrocytic SOD preparation was tested it showed the same cell inhibitory activities as p16. These observations show that superoxide dismutase is able to affect the cycling and growth factor responses of hematopoietic cells, activities that have not previously been associated with this enzyme.
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1 September 1990
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September 01 1990
Purification of an inhibitor of erythroid progenitor cell cycling and antagonist to interleukin 3 from mouse marrow cell supernatants and its identification as cytosolic superoxide dismutase.
F G Pluthero,
F G Pluthero
Department of Anatomy, University of Toronto, Ontario, Canada.
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M Shreeve,
M Shreeve
Department of Anatomy, University of Toronto, Ontario, Canada.
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D Eskinazi,
D Eskinazi
Department of Anatomy, University of Toronto, Ontario, Canada.
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H van der Gaag,
H van der Gaag
Department of Anatomy, University of Toronto, Ontario, Canada.
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K S Huang,
K S Huang
Department of Anatomy, University of Toronto, Ontario, Canada.
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J D Hulmes,
J D Hulmes
Department of Anatomy, University of Toronto, Ontario, Canada.
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M Blum,
M Blum
Department of Anatomy, University of Toronto, Ontario, Canada.
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A A Axelrad
A A Axelrad
Department of Anatomy, University of Toronto, Ontario, Canada.
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F G Pluthero
Department of Anatomy, University of Toronto, Ontario, Canada.
M Shreeve
Department of Anatomy, University of Toronto, Ontario, Canada.
D Eskinazi
Department of Anatomy, University of Toronto, Ontario, Canada.
H van der Gaag
Department of Anatomy, University of Toronto, Ontario, Canada.
K S Huang
Department of Anatomy, University of Toronto, Ontario, Canada.
J D Hulmes
Department of Anatomy, University of Toronto, Ontario, Canada.
M Blum
Department of Anatomy, University of Toronto, Ontario, Canada.
A A Axelrad
Department of Anatomy, University of Toronto, Ontario, Canada.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1990) 111 (3): 1217–1223.
Citation
F G Pluthero, M Shreeve, D Eskinazi, H van der Gaag, K S Huang, J D Hulmes, M Blum, A A Axelrad; Purification of an inhibitor of erythroid progenitor cell cycling and antagonist to interleukin 3 from mouse marrow cell supernatants and its identification as cytosolic superoxide dismutase.. J Cell Biol 1 September 1990; 111 (3): 1217–1223. doi: https://doi.org/10.1083/jcb.111.3.1217
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