The expression of mRNAs for the RI alpha, RII alpha, and C alpha subunits of cAMP-dependent protein kinase has been studied in different ram germ cells. The sizes of the specific RI alpha, RII alpha, and C alpha mRNAs, observed in germ cells were 1.6, 2.0, and 2.6 kb, respectively. RI alpha and C alpha mRNAs were mainly expressed in primary spermatocytes. A postmeiotic expression predominating in early spermatids was unique to RII alpha mRNA. The location of RI, RII alpha, and C subunits in well-defined organelles of ram spermatids and epididymal sperm was assessed by immunogold electron microscopy. In spermatids, RI, RII alpha, and C were essentially present in the forming acrosome and, to a lesser extent, in the nucleus. During sperm epididymal maturation, the protein kinases disappeared from the acrosome and were detected in a variety of sperm functional areas, such as the tip of the acrosome, the motility apparatus, and the membrane network. The present study on subunits of cAMP-dependent protein kinase supports the concept that specific functions are attached to the different subunits in that it shows differential expression and differential subcellular localization in germ cells.
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1 September 1989
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September 01 1989
Differential expression and subcellular localization for subunits of cAMP-dependent protein kinase during ram spermatogenesis.
C Pariset,
C Pariset
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
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J Feinberg,
J Feinberg
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
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J L Dacheux,
J L Dacheux
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
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O Oyen,
O Oyen
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
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T Jahnsen,
T Jahnsen
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
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S Weinman
S Weinman
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
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C Pariset
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
J Feinberg
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
J L Dacheux
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
O Oyen
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
T Jahnsen
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
S Weinman
Faculté de Médecine Saint-Antonie, Université Pierre et Marie Curie, Paris.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1989) 109 (3): 1195–1205.
Citation
C Pariset, J Feinberg, J L Dacheux, O Oyen, T Jahnsen, S Weinman; Differential expression and subcellular localization for subunits of cAMP-dependent protein kinase during ram spermatogenesis.. J Cell Biol 1 September 1989; 109 (3): 1195–1205. doi: https://doi.org/10.1083/jcb.109.3.1195
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