The tissue distribution of type II and type IX collagen in 17-d-old chicken embryo was studied by immunofluorescence using polyclonal antibodies against type II collagen and a peptic fragment of type IX collagen (HMW), respectively. Both proteins were found only in cartilage where they were co-distributed. They occurred uniformly throughout the extracellular matrix, i.e., without distinction between pericellular, territorial, and interterritorial matrices. Tissues that undergo endochondral bone formation contained type IX collagen, whereas periosteal and membranous bones were negative. The thin collagenous fibrils in cartilage consisted of type II collagen as determined by immunoelectron microscopy. Type IX collagen was associated with the fibrils but essentially was restricted to intersections of the fibrils. These observations suggested that type IX collagen contributes to the stabilization of the network of thin fibers of the extracellular matrix of cartilage by interactions of its triple helical domains with several fibrils at or close to their intersections.
Skip Nav Destination
Article navigation
1 May 1986
Article|
May 01 1986
On the role of type IX collagen in the extracellular matrix of cartilage: type IX collagen is localized to intersections of collagen fibrils.
W Müller-Glauser
B Humbel
M Glatt
P Sträuli
K H Winterhalter
P Bruckner
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1986) 102 (5): 1931–1939.
Citation
W Müller-Glauser, B Humbel, M Glatt, P Sträuli, K H Winterhalter, P Bruckner; On the role of type IX collagen in the extracellular matrix of cartilage: type IX collagen is localized to intersections of collagen fibrils.. J Cell Biol 1 May 1986; 102 (5): 1931–1939. doi: https://doi.org/10.1083/jcb.102.5.1931
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
Advertisement