Concanavalin A (Con A) has been used to activate platelets, inducing a specific interaction between the glycoprotein IIb-IIIa complex and the cytoskeleton of the activated platelet. In agreement with this, we have shown that Con A activates human platelets, initiating phosphorylation, secretion, and cytoskeletal formation. Con A and cytochalasin B were used to demonstrate a reciprocal interaction of the glycoprotein complex with the platelet cytoskeleton. Additionally, we have shown that a similar reciprocity is provided by the multivalent fibrin-fibrinogen platelet interaction found in the thrombin-induced clot. Con A differs from other activators in precipitating an apparent cytoskeletal core despite a complete inhibition of platelet activation by prostaglandin E1. We suggest, from this result, that Con A may be cross-linking a membrane-associated cytoskeletal complex present in the unactivated platelet.
Skip Nav Destination
Article navigation
1 September 1985
Article|
September 01 1985
Reciprocal transmembranous receptor-cytoskeleton interactions in concanavalin A-activated platelets.
M E Wheeler
J M Gerrard
R C Carroll
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1985) 101 (3): 993–1000.
Citation
M E Wheeler, J M Gerrard, R C Carroll; Reciprocal transmembranous receptor-cytoskeleton interactions in concanavalin A-activated platelets.. J Cell Biol 1 September 1985; 101 (3): 993–1000. doi: https://doi.org/10.1083/jcb.101.3.993
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement