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Journal Articles
The Journal of General Physiology (2020) 152 (3): e201912520.
Published: 27 January 2020
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Reversal of mouse cone photocurrent. (A  –D  )  Whole-cell patch recordings...
Published: 27 January 2020
Figure 1. Reversal of mouse cone photocurrent. (A –D ) Whole-cell patch recordings from Gnat1 −/− mice (A and B) and from Cx36 −/− mice (C and D). Holding potential was initially −50 mV and was changed to test potentials from −90 mV to +70 mV in 20-mV steps. Sample command step shown in ... More
Images
Voltage-gated Ca 2+  and Ca 2+ -activated Cl −  currents.  Whole-cell patch...
Published: 27 January 2020
Figure 2. Voltage-gated Ca 2+ and Ca 2+ -activated Cl − currents. Whole-cell patch recordings from Cx36 −/− cones. Recordings were made with a Cs + internal (pipette) solution, which contained both Cs + and TEA in sufficient concentrations to block BK channels (see Materials and methods).... More
Images
Feedback-mediated currents elicited by light after dialysis of GTP-γ-S. (A)...
Published: 27 January 2020
Figure 3. Feedback-mediated currents elicited by light after dialysis of GTP-γ-S. (A) Examples from three different cones from Cx36 −/− mice show the effect of introducing 2 mM GTP-γ-S from the whole-cell patch pipette into the cone cell body. Currents were recorded at a holding potential of ... More
Images
i h  (HCN channels).  Whole-cell patch recordings from  Cx36 −/−   cones wi...
Published: 27 January 2020
Figure 4. i h (HCN channels). Whole-cell patch recordings from Cx36 −/− cones with 25 mM TEA and 10 µM ISR included in the bath solution. The pipette solution was K-Asp. (A) Currents from a sample cone held at −50 mV and stepped for 400 ms to potentials of −25 to −135 mV in increments of 1... More
Images
Voltage-gated K +  currents.  Whole-cell patch recordings from  Cx36 −/−   ...
Published: 27 January 2020
Figure 5. Voltage-gated K + currents. Whole-cell patch recordings from Cx36 −/− cones. (A) Voltage-gated transient current (i k,trans ) recorded in the presence of 25 mM TEA to block a sustained current, together with 10 µM ISR to block Ca 2+ currents and Ca 2+ -activated K + and Cl − c... More
Journal Articles
The Journal of General Physiology (2020) 152 (6): e201912498.
Published: 24 January 2020
Images
Derivatization of WGA with a small-molecule fluorescent pH sensor and cell ...
Published: 24 January 2020
Figure 1. Derivatization of WGA with a small-molecule fluorescent pH sensor and cell surface labeling to visualize plasma membrane proton fluxes. (A) Synthetic scheme of WGA-pHRho. Using the degree of labeling equation (Materials and methods) on average, one WGA monomer is labeled with one pHRh... More
Images
WGA labeling and voltage-clamp fluorometry of CHO cells expressing voltage-...
Published: 24 January 2020
Figure 2. WGA labeling and voltage-clamp fluorometry of CHO cells expressing voltage-gated channels. (A) hHv-1 (top) and Shaker-IR (bottom) currents were recorded before (left) and after (right) WGA labeling (50 µg/ml). Middle panels are currents before (black) and after (red) WGA wash-in at 60 ... More
Images
Characterization of pH-sensitive WGA conjugates, related to    Fig. 2   .  ...
Published: 24 January 2020
Figure S1. Characterization of pH-sensitive WGA conjugates, related to Fig. 2 . (A) Fluorescence-pH plot of WGA-pHRho in solution; excitation wavelength, 550 nm. a.u., arbitrary units. (B) ΔF/F 0 -I plot of WGA-pHRho (red), homemade WGA-fluorescein (dark green), and WGA-fluorescein from ... More
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Changes in WGA-pHRho fluorescence requires exogenous expression of proton c...
Published: 24 January 2020
Figure S2. Changes in WGA-pHRho fluorescence requires exogenous expression of proton channels in CHO cells. Related to Fig. 2 . (A and B) Current–voltage relationships for (A) Hv-1 and (B) Shaker-IR (R362H/W434F) expressed in CHO cells. Hv-1 was held at −80 mV, and currents were elicited f... More
Images
Cell surface pH calibration of WGA-pHRho and WGA-fluorescein.  (A)  CHO cel...
Published: 24 January 2020
Figure 3. Cell surface pH calibration of WGA-pHRho and WGA-fluorescein. (A) CHO cells were labeled with either WGA-fluorescein or WGA-pHRho, and the change of fluorescence (ΔF/F 0 %) was plotted using pH standards (HEPES; 1 mM, open symbols; 10 mM, closed symbols); F o was chosen to be at the ... More
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Structured light microscopy of lactate-stimulated proton transport in rat v...
Published: 24 January 2020
Figure 4. Structured light microscopy of lactate-stimulated proton transport in rat ventricular myocytes labeled with WGA-pHRho. (A) A ∼750-nm illuminated z -plane of a WGA-pHRho–labeled ventricular myocyte ∼2 μm above the coverslip focal plane. (B) Time course of 10 mM lactate perfusion befo... More
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Proton efflux from primary neuron–astrocyte cocultures labeled with WGA-pHR...
Published: 24 January 2020
Figure 5. Proton efflux from primary neuron–astrocyte cocultures labeled with WGA-pHRho. (A) Merged Airyscan image of the neuronal and astrocyte planes labeled with WGA-pHRho (red) and DAPI (blue). (B) Pseudocolor image showing the averaged ΔF/F 0 of the regions of interest (ROI) in A after ... More
Journal Articles
The Journal of General Physiology (2020) 152 (3): e201811998.
Published: 23 January 2020
Images
LC15 transgenic mice exhibit robust CUG exp  RNA expression in the heart  ....
Published: 22 January 2020
Figure 1. LC15 transgenic mice exhibit robust CUG exp RNA expression in the heart . (A) Schematic of transgene used to generate LC15 mice. An uninterrupted expanded CTG-repeat tract generated using a cell-free assembly procedure described previously ( Osborne and Thornton, 2008 ) was embedded... More
Images
Mbnl1 and Mbnl2 expression levels in hearts from LC15 mice.  (A)  Represent...
Published: 22 January 2020
Figure S1. Mbnl1 and Mbnl2 expression levels in hearts from LC15 mice. (A) Representative Western blot of Mbnl1 (top) and gapdh (bottom) in lysates from ventricular myocytes from WT and LC15 mice. (B) Relative Mbnl1 band intensity (normalized to gapdh) for lysates from ventricular myocytes is... More
Images
ECG analyses in anesthetized WT and LC15 mice.  (A)  Representative signal-...
Published: 22 January 2020
Figure 2. ECG analyses in anesthetized WT and LC15 mice. (A) Representative signal-averaged ECG traces aligned to the start of the Q-wave from 3-mo-old WT ( n = 11 mice; black) and LC15 ( n = 14 mice; red) littermate mice. The traces reflect the average of 3 s of continuous ECG recording from... More
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ECG analyses of conscious WT and LC15 mice using implanted radiotelemeters....
Published: 22 January 2020
Figure 3. ECG analyses of conscious WT and LC15 mice using implanted radiotelemeters. ECGs were recorded continuously for 60 h in mice surgically implanted with radiotelemetry devices (ETA-F10). (A) Plot of average heart rate (HR) over 5-min intervals during the 60 h of recording for a cohort ... More
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LC15 mice exhibited increased sensitivity for arrhythmias following acute f...
Published: 22 January 2020
Figure 4. LC15 mice exhibited increased sensitivity for arrhythmias following acute flecainide administration. (A) Representative 1-s ECG recordings obtained from WT and LC15 mice immediately before (left), 2 min after (middle), and 5 min after (right) flecainide administration (20 mg/kg intrap... More