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Journal Articles

A proteome-wide yeast degron collection for the dynamic study of protein function

Rosario Valenti, Yotam David, Dunya Edilbi, Benjamin Dubreuil, Angela Boshnakovska, Yeynit Asraf, Tomer-Meir Salame, Ehud Sass, Peter Rehling, Maya Schuldiner
Journal: Journal of Cell Biology
J Cell Biol (2024) 224 (2): e202409050.
https://doi.org/10.1083/jcb.202409050
Published: 18 December 2024
View article titled, A proteome-wide yeast degron collection for the dynamic study of protein function
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Includes: Supplementary data
Journal Articles

Genome-wide conditional degron libraries for functional genomics

Eduardo Gameiro, Karla A. Juárez-Núñez, Jia Jun Fung, Susmitha Shankar, Brian Luke, Anton Khmelinskii
Journal: Journal of Cell Biology
J Cell Biol (2024) 224 (2): e202409007.
https://doi.org/10.1083/jcb.202409007
Published: 18 December 2024
View article titled, Genome-wide conditional degron libraries for functional genomics
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Includes: Supplementary data
Journal Articles

One step 4× and 12× 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures

Roshan X. Norman, Yu-Chia Chen, Emma E. Recchia, Jonathan Loi, Quincy Rosemarie, Sydney L. Lesko, Smit Patel, Nathan Sherer, Motoki Takaku, Mark E. Burkard, Aussie Suzuki
Journal: Journal of Cell Biology
J Cell Biol (2024) 224 (2): e202407116.
https://doi.org/10.1083/jcb.202407116
Published: 03 December 2024
View article titled, One step 4× and 12× 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures
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Journal Articles

Visualizing ER-phagy and ER architecture in vivo

Yongjuan Sang, Boran Li, Tinglin Su, Hanyu Zhan, Yue Xiong, Zhiming Huang, Changjing Wang, Xiaoxia Cong, Mengjie Du, Yang Wu, Hang Yu, Xi Yang, Kezhi Ding, Xuhua Wang, Xiaolong Miao, Weihua Gong, Liang Wang, Jingwei Zhao, Yiting Zhou, Wei Liu, Xinyang Hu, Qiming Sun
Journal: Journal of Cell Biology
J Cell Biol (2024) 223 (12): e202408061.
https://doi.org/10.1083/jcb.202408061
Published: 18 November 2024
View article titled, Visualizing ER-phagy and ER architecture in vivo
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Includes: Supplementary data
Journal Articles

Definition of phosphatidylinositol 4,5-bisphosphate distribution by freeze-fracture replica labeling

Takuma Tsuji, Junya Hasegawa, Takehiko Sasaki, Toyoshi Fujimoto
Journal: Journal of Cell Biology
J Cell Biol (2024) 224 (1): e202311067.
https://doi.org/10.1083/jcb.202311067
Published: 04 November 2024
View article titled, Definition of phosphatidylinositol 4,5-bisphosphate distribution by freeze-fracture replica labeling
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Includes: Supplementary data
Journal Articles

Heterogeneity of late endosome/lysosomes shown by multiplexed DNA-PAINT imaging

Charles Bond, Siewert Hugelier, Jiazheng Xing, Elena M. Sorokina, Melike Lakadamyali
Journal: Journal of Cell Biology
J Cell Biol (2024) 224 (1): e202403116.
https://doi.org/10.1083/jcb.202403116
Published: 01 November 2024
View article titled, Heterogeneity of late endosome/lysosomes shown by multiplexed DNA-PAINT imaging
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Includes: Supplementary data
Journal Articles

MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans

Xiaofan Liu, Zhi Chang, Pingping Sun, Beibei Cao, Yuzhi Wang, Jie Fang, Yechun Pei, Baohui Chen, Wei Zou
Journal: Journal of Cell Biology
J Cell Biol (2024) 224 (1): e202403198.
https://doi.org/10.1083/jcb.202403198
Published: 14 October 2024
View article titled, MONITTR allows real-time imaging of transcription and endogenous proteins in <em>C. elegans</em>
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Includes: Supplementary data
Journal Articles

Arrayed CRISPRi library to suppress genes required for Schizosaccharomyces pombe viability

Ken Ishikawa, Saeko Soejima, Takashi Nishimura, Shigeaki Saitoh
Journal: Journal of Cell Biology
J Cell Biol (2024) 224 (1): e202404085.
https://doi.org/10.1083/jcb.202404085
Published: 08 October 2024
View article titled, Arrayed CRISPRi library to suppress genes required for <em>Schizosaccharomyces pombe</em> viability
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Includes: Supplementary data
Journal Articles

Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells

Miharu Maeda, Masashi Arakawa, Yukie Komatsu, Kota Saito
Journal: Journal of Cell Biology
J Cell Biol (2024) 223 (10): e202403179.
https://doi.org/10.1083/jcb.202403179
Published: 05 August 2024
View article titled, Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells
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Includes: Supplementary data
Journal Articles

A fluorogenic complementation tool kit for interrogating lipid droplet–organelle interaction

Xiao Li, Rico Gamuyao, Ming-Lun Wu, Woo Jung Cho, Sharon V. King, R.A. Petersen, Daniel R. Stabley, Caleb Lindow, Leslie K. Climer, Abbas Shirinifard, Francesca Ferrara, Robert E. Throm, Camenzind G. Robinson, Yiwang Zhou, Alexandre F. Carisey, Alison G. Tebo, Chi-Lun Chang
Journal: Journal of Cell Biology
J Cell Biol (2024) 223 (9): e202311126.
https://doi.org/10.1083/jcb.202311126
Published: 01 July 2024
View article titled, A fluorogenic complementation tool kit for interrogating lipid droplet–organelle interaction
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Includes: Supplementary data
Journal Articles

Intraflagellar transport speed is sensitive to genetic and mechanical perturbations to flagellar beating

In Special Collection:
Centrosomes and Cilia 2024
Sophie Gray, Cecile Fort, Richard John Wheeler
Journal: Journal of Cell Biology
J Cell Biol (2024) 223 (9): e202401154.
https://doi.org/10.1083/jcb.202401154
Published: 03 June 2024
View article titled, Intraflagellar transport speed is sensitive to genetic and mechanical perturbations to flagellar beating
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Includes: Supplementary data
Journal Articles

Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides

In Special Collection:
The Year in Cell Biology: 2024
Hannes Maib, Petia Adarska, Robert Hunton, James H. Vines, David Strutt, Francesca Bottanelli, David H. Murray
Journal: Journal of Cell Biology
J Cell Biol (2024) 223 (6): e202310095.
https://doi.org/10.1083/jcb.202310095
Published: 05 April 2024
View article titled, Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides
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Includes: Supplementary data
Journal Articles

Identification of small molecule inhibitors of G3BP-driven stress granule formation

Brian D. Freibaum, James Messing, Haruko Nakamura, Ugur Yurtsever, Jinjun Wu, Hong Joo Kim, Jeff Hixon, Rene Marc Lemieux, Jay Duffner, Walter Huynh, Kathy Wong, Michael White, Christina Lee, Rachel E. Meyers, Roy Parker, J. Paul Taylor
Journal: Journal of Cell Biology
J Cell Biol (2024) 223 (3): e202308083.
https://doi.org/10.1083/jcb.202308083
Published: 29 January 2024
View article titled, Identification of small molecule inhibitors of G3BP-driven stress granule formation
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Includes: Supplementary data
Journal Articles

Membrane contact site detection (MCS-DETECT) reveals dual control of rough mitochondria–ER contacts

In Special Collection:
Inter-Organelle Contacts , Structural Biology 2024 , Tools Collection 2024
Ben Cardoen, Kurt R. Vandevoorde, Guang Gao, Milene Ortiz-Silva, Parsa Alan, William Liu, Ellie Tiliakou, A. Wayne Vogl, Ghassan Hamarneh, Ivan R. Nabi
Journal: Journal of Cell Biology
J Cell Biol (2023) 223 (1): e202206109.
https://doi.org/10.1083/jcb.202206109
Published: 10 November 2023
View article titled, Membrane contact site detection (MCS-DETECT) reveals dual control of rough mitochondria–ER contacts
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Includes: Supplementary data
Journal Articles

Inducible tricolor reporter mouse for parallel imaging of lysosomes, mitochondria, and microtubules

Vera Hutchison, Anne Lynch, Andrés Mauricio Gutierrez-Gamez, Jichao Chen
Journal: Journal of Cell Biology
J Cell Biol (2023) 223 (1): e202305086.
https://doi.org/10.1083/jcb.202305086
Published: 02 November 2023
View article titled, Inducible tricolor reporter mouse for parallel imaging of lysosomes, mitochondria, and microtubules
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Includes: Supplementary data
Journal Articles

Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy

Elise Delage, Thomas Guilbert, Frank Yates
Journal: Journal of Cell Biology
Series: Reproducibility
J Cell Biol (2023) 222 (11): e202307143.
https://doi.org/10.1083/jcb.202307143
Published: 17 October 2023
View article titled, Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy
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Journal Articles

The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ

Lukas A. Fuentes, Zach Marin, Jonathan Tyson, David Baddeley, Joerg Bewersdorf
Journal: Journal of Cell Biology
J Cell Biol (2023) 222 (10): e202301112.
https://doi.org/10.1083/jcb.202301112
Published: 26 July 2023
View article titled, The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ
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Includes: Supplementary data
Journal Articles

A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle

In Special Collection:
Tools Collection 2024
Klara I. Jansen, Malina K. Iwanski, Mithila Burute, Lukas C. Kapitein
Journal: Journal of Cell Biology
J Cell Biol (2023) 222 (5): e202106105.
https://doi.org/10.1083/jcb.202106105
Published: 02 March 2023
View article titled, A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle
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Journal Articles

Rapid profiling of DNA replication dynamics using mass spectrometry–based analysis of nascent DNA

Mohamed E. Ashour, Andrea K. Byrum, Alice Meroni, Jun Xia, Saurabh Singh, Roberto Galletto, Susan M. Rosenberg, Alessandro Vindigni, Nima Mosammaparast
Journal: Journal of Cell Biology
J Cell Biol (2023) 222 (4): e202207121.
https://doi.org/10.1083/jcb.202207121
Published: 16 February 2023
View article titled, Rapid profiling of DNA replication dynamics using mass spectrometry–based analysis of nascent DNA
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Includes: Supplementary data
Journal Articles

Semi-automated 3D fluorescence speckle analyzer (3D-Speckler) for microscope calibration and nanoscale measurement

Jonathan Loi, Xiaofei Qu, Aussie Suzuki
Journal: Journal of Cell Biology
J Cell Biol (2023) 222 (4): e202202078.
https://doi.org/10.1083/jcb.202202078
Published: 30 January 2023
View article titled, Semi-automated 3D fluorescence speckle analyzer (3D-Speckler) for microscope calibration and nanoscale measurement
Open the PDF for Semi-automated 3D fluorescence speckle analyzer (3D-Speckler) for microscope calibration and nanoscale measurement in another window
Includes: Supplementary data